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Positioned on an elevated and narrow 50 cm beam, the mice were given a maximum of 60 s to cross it

Baseline measurements before starting, periodic monitoring during use, and follow-up assessment after cycling off provide data points that can reveal individual response patterns

Western immunoblot analyses For the preparation of total liver homogenates, frozen liver pieces were homogenized in RIPA buffer (150 mM NaCl, 1% TritonX-100, 0.25% sodium deoxycholate, 0.1% SDS, 50 mM Tris, 1 mM EDTA, 1 mM PMSF, protease inhibitor cocktail, pH 7.4) on ice with a tissue tearor (BioSpec Products, Bartlesville)

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